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    • 专利摘要:
    method for minimizing enzyme-based aerosol mist using a pressure spray system. the methods of the present invention are presented to improve the safety and application of commercial applications of cleaning compositions that include enzymes and other irritating proteins. the methods reduce protein mist and aerosolization so that inhalation and exposure to the same are reduced. in accordance with the invention, when commercial pressurized sprinklers are used to apply the use cleaning compositions containing protein up to 5 ppm protein, aerosolization is decreased to below 60 ng active protein per cubic meter. Applicants have also identified that a specific metering nozzle/tip, dose rate and low pressure application of no more than 100 psi are critical to achieving the benefits of the invention.
    公开号:BR112016025205B1
    申请号:R112016025205-5
    申请日:2015-04-27
    公开日:2021-08-31
    发明作者:Nathan D. Peitersen；Charles Allen Hodge；Terrance P. Everson；Stephen James Engel
    申请人:Ecolab Usa Inc.；
    IPC主号:
    专利说明:

    Field of Invention
    [001] The invention relates to methods and practices for the safe application of chemical compositions containing enzymes or other proteins, applied through pressurized devices such as pumps or sprinklers. Aerosolization of proteins can pose a health hazard if proteins become present in the air and are ingested by users. The methods are particularly adapted for using pressurized delivery devices that load and apply such compositions in commercial applications. Background of the invention
    [002]Aqueous sprayable compositions can be applied to a hard surface with a temporary trigger spray device or an aerosol spray device. These compositions are very useful because they can be applied by spraying to vertical, suspended or inclined surfaces. Spray devices create a spray pattern of the water sprayable composition that comes into contact with targeted hard surfaces. Most sprayable compositions come to reside on the target surface as large sprayed deposits, while a small portion of the sprayable composition can become an airway-borne aerosol or mist, which consists of small particles of the cleaning composition that may remain suspended or dispersed in the atmosphere surrounding the dispersion site for a period of time such as between about 5 seconds to about 10 minutes. Suspension and dispersion make these particles available for ingestion by the user and can pose a health hazard, particularly if proteins or other enzymes are inhaled.
    [003] Enzymes are important constituents in modern detergent products. They are proteins that catalyze chemical reactions and break down dirt and stains. Enzymes are allergens and can cause respiratory allergies similar to other allergens such as pollen, mites and animal hair. When allergens are inhaled in the form of dust or aerosols they can give rise to the formation of specific antibodies which can result in sensitization of the immune system. Upon re-exposure, people can develop respiratory allergies with symptoms similar to those of asthma and hay fever. These symptoms may include itching and redness of the mucous membranes, watery/runny nose, sneezing, nasal or bell congestion, hoarseness from shortness of breath, coughing, and tightness in the chest.
    [004] Proteolytic enzymes can cause eye irritation and skin irritation.
    [005] Long-term exposure to these irritants through repetitive applications can cause significant problems. Often, after breathing in the finely divided aerosol or mist, an irrepressible and very strong choking response is seen in most individuals who come into contact with irritating proportions of the aerosol produced by typical sprayed cleaners. The choking response is inconvenient, reduces cleaning efficiency in a variety of applications, and in sensitive individuals can cause asthma attacks, respiratory distress or other discomfort or injury.
    [006] It was thought, in general, that reducing the aerosolization of enzymes involves increasing the viscosity of solutions or is limited to the application of only naturally viscous solutions. Enzyme aerosolization, however, is dependent on a number of different parameters, eg formulation, product enzyme concentration, consumer habits and practices, and injector device. High viscosity formulations and foam sprays are designed to generate less enzyme exposure than low viscosity liquid formulations.
    [007] Applicants have identified methods for the application of thin water and other solutions containing low viscosity enzyme, thus reducing the proteins present in any aerosol carried by airways or mist associated with it. The following Sumerian is given by way of example, but this is not a limitation. It is merely provided to help the reader understand some aspects of the invention. Invention Summary
    [008] Applicants have identified particular application methods for use in industrial and commercial spray systems that reduce haze and aerosolization of proteins present in cleaning solutions. This will put less danger to the health of janitors and other professionals who use these buckets and solutions on a recurring basis. The reduction in health hazard will result in fewer lost work days, improved efficiency and less discomfort for employees.
    [009] According to the invention, when commercial pressurized spray systems are used to apply cleaning compositions that employ protein or other irritants that can become aerosolized, the low pressure application needs to be used, preferably no longer that 689.4 kPa (100 psi). Applicants have also identified a specific injector, (one that delivers a particle size of 750 microns) and application (56.7 g per 3.69 liters (2 ounces per gallon) of 0.1 to 10% by weight of protein in a concentrated solution or approximately 5 ppm protein in a use solution) critical for the method as a whole.
    [010] The method is particularly adapted for commercial spray devices such as those described in US patent publications US2007/0187528 and US2012/0312390, which descriptions are incorporated in their entirety into the present invention by reference. Applicants tested a spray device with various cleaning/sanitizing formations including enzyme lipase to verify critical parameters that reduce aerosolization of this protein.
    [011]According to the invention, the applicant has found that the use of the spray gun depicted here with the system dispensed at a rate of 2 oz. Per gallon, at a pressure of at least 25 and preferably less than 689.4 kPa (100 psi), more preferably less than 517.1 kPa (75 psi) with up to 0.003% weight percent protein in the solution of use (or 3 ppm) will be dispensed in a safe manner.
    [012] Therefore, it is an object of the present invention to increase cleaning efficiency and safety by using a low pressure pump to apply the appropriate amount of cleaning solution and to prevent protein aerosolization and provide a fully self-powered unit portable to help clean and sanitize commercial kitchens and toilets.
    [013] The above aspects and others will become evident from the following detailed description of the invention when considered in conjunction with the attached figures.
    [014] While various embodiments are disclosed, still other embodiments of the present invention will become apparent to those skilled in the art from the following detailed description showing and describing the illustrative embodiments of the invention. Consequently, the detailed description and figures can be considered illustrative in nature and not restrictive.
    [015] Surprisingly, applicants were able to reduce aerosolization without the need for traditional anti-mist components such as polyethylene oxide, polyacrylamide, polyacrylate and combinations thereof, see for example publication US 20130255729. In a preferred embodiment the methods of the invention employ compositions that are substantially free of anti-mist components such as polyethylene oxide, polyacrylamide and polyacrylate. Brief description of the drawings
    [016] FIG. 1 is a right side front perspective view of one embodiment of a commercial pressurized spray cleaner which may be used in accordance with the invention.
    [017] FIG. 2 is a left side rear perspective view of the embodiment of FIG. 1.
    [018] FIG. 3 is a right front perspective view of the embodiment of FIGS. 1 and 2 with the front faceplate and holders removed.
    [019] FIG. 4 is a non-limiting diagrammatic representation of a typical spray gun that can be used in the method of the invention.
    [020] FIG. 5 is a non-limiting diagrammatic representation of a typical spray gun for attaching the spray gun shown in FIG. 4 and used in exams. Detailed description of preferred modalities.
    [021] Other than the working examples or where otherwise indicated, all numbers expressing amounts of ingredients or reaction conditions used in the present invention are to be understood as being modified in all cases by the term "about in".
    [022] As used herein, percent by weight (% by weight), percent by weight, % by weight, and the like are synonyms that refer to concentrations of a substance as the weight of that substance divided by the total weight of the composition and multiplied by 100.
    [023] As used herein, the term "about" modifying the amount of an ingredient in the compositions of the invention or employed in the methods of the invention refers to variation in the numerical amount that can occur, for example, through a typical measurement and liquid handling procedures used to make concentrates or solutions for real-world use; through inadvertent error in these procedures; through differences in the manufacture, source or purity of the ingredients used to make the compositions or carry out the methods; and the like. The term about also encompasses amounts that differ due to different equilibrium conditions for a composition resulting from a specific initial mixture. Modified or not by the term "about", the claims include equivalents for the amounts.
    [024]Cleaning means to carry out or assist in removing dirt, bleaching, reducing the population of microbes, rinsing, or combinations thereof.
    [025]It should be noted that, as used in this descriptive report and the appended claims, the singular forms “a”, “an”, “the” and “a” include plural references unless the content dictates clearly the opposite. Thus, for example, reference to a composition containing “a compound” includes a mixture of two or more compounds. It should also be noted that the term “or” is generally used in its sense to include “and/or” unless the content clearly states otherwise.
    [026] The term "actives" or "active percentages" or "weight percentage of actives" or "actives concentration" are used interchangeably in the present invention and refer to the concentration of those ingredients involved in cleaning expressed as a percentage of less inert ingredients like water or salts.
    [027] As used herein, the term "substantially free" refers to compositions completely lacking the component or having a small amount of the component that the component does not affect the effectiveness of the composition. The component can be present as an impurity or as a contaminant and must be less than 0.5% by weight. In another embodiment, the amount of component is less than 0.1% by weight and in yet another embodiment, the amount of component is less than 0.01% by weight.
    [028] Applicants have identified specific application methods for use in spray devices employed in commercial cleaning that reduce haze and aerosolization of proteins present in certain cleaning solutions. Applicants' methods can be used to employ spray wash cleaning systems with chemical formulas including up to 5% by weight, preferably up to 1.0% by weight, and more preferably up to 0.5% by weight of protein in a concentrated solution that is diluted to a use solution of 56.7 g per 3.69 liters (2 ounces per gallon) of water. In an in-use solution applied through a 56.7 g per 3.69 liter (2 oz per gallon) conveyor system, the amount of protein present that was safely applied was approximately 0.0016% w /w, this is about half the acceptable limit of aerosolized enzyme, so at a use concentration the invention includes up to 0.003% enzyme or 3 ppm.
    [029] According to the invention, low pressure commercial buckets (689.4 kPa (100 psi) or less) are used to apply cleaning compositions that include enzymes and other proteins or other irritants. Threshold levels during the cycle need to be below 60 ng of active protein per cubic meter. Applicants have also identified a specific mouthpiece useful for the method as a to do. In accordance with the invention, a suitable spray nozzle is used to dispense a dilution of a concentrated solution of up to 3 ppm protein at a rate of 1.89 liters per minute (0.5 gallons per minute) of use. solution. A sprinkler nozzle that produces an average particle size with a diameter of 1500 microns like the 25 degree angle 1/4” MEG 25035 Flat Jet Sprinkler Systems of nozzle capacity makes it possible for commercial sprinkler systems to apply compositions without aerosolization for proteins. For the examples of the present invention, the 1/4” MEG 25035 nozzle, the nozzle has a 1/4 inch inlet diameter for a 25 degree spray angle at a capacity of 0.35 gallons per minute at 275, 7 kPa (40 psi). This application from about 0.3 gpm to about 4 gpm. This equates to about 675 microns for a median volume diameter of the spray particles. In general, the higher the pressure and the smaller the nozzle orifice, the smaller the particles. The invention is not limited to this specific nozzle as other nozzles may apply the same particle size, such as a larger orifice at a higher pressure or a smaller orifice at a lower pressure, and there may be different geometries for the spray instead. of the spray at a 25 degree flat angle. To apply cleaners to floors, the application would be from about 0.1 gpm to about 5 gpm.
    [030] Methods particularly adapted for spray transport such as those described in US patent publications US2007/0187528 and US2012/0312390 whose descriptions are incorporated in their entirety into the present invention by reference. Applicants have tested spray carriers which are not designed or contemplated to be used for application of solutions that include proteins and surprisingly found that after appropriate modification of the process, the method can be adapted to allow for the use of formulations containing enzyme without its aerosolization.
    [031] The invention presents a means of sanitizing toilets that makes the cleaning process faster, more effective and more efficient by reducing overspray and waste using a low pressure pump to apply the correct amount of cleaning solution and also so that any enzymes or proteins present in said cleaning solution are not aerosolized. The device can also employ a rechargeable battery, reducing setup time and allowing the unit to be used in installations that do not have electrical outlets. In addition, the apparatus is equipped with a low pressure spray release system that is designed to dispense the proper amount of cleaning solution eliminating oversaturation and waste, saving water and chemicals, and increasing efficiency by reducing configuration and recovery time. In accordance with the invention, applicants have found that using the spray nozzle depicted in the present invention with the system dispensed at a rate of 56.7 g per 3.69 liters (2 ounces per gallon), with a pressure of 517.1 kPa (75 psi) solutions with up to 0.2 weight percent protein in the original concentrated solution (diluted to 56.7 g per 3.69 liters (2 ounces per gallon) or up to 3 ppm % by weight of enzyme will be safely dispensed.
    [032] In a preferred embodiment, a low pressure sprinkler transport system is employed for the methods of the invention as described below.
    [033] Referring now to Figure 1, an embodiment 10 is shown in front and right side views and featuring a base 11 and a face plate 20. The base 11 of the janitorial bucket 10 contains a hollow space in the base 11 used as a freshwater reservoir 12.
    [034] The rear of the base 11 extends upwards along the rear of figure 1 in a one-piece structure to form a cable 36 and to give a general shape to the wheelbarrow 10. Attached to the outer bottom of the base 11 in the present embodiment are two fixed axle rear wheels 14 and two freely swiveling front wheels 16. Front wheels 16 are released to complete 360 degrees of rotation, facilitating better bucket control and steering. To provide a simple and efficient means of draining the fresh water reservoir, the apparatus 10 has been equipped with a drain chute 18. The drain chute 18 is located on the base 11 below the face plate 20 and between the two front wheels. 16.
    [035]Mode 10 contains a removable face plate 20. FIG. 3 shows a view of the apparatus 10 with the face plate 20 (figure 1) removed. Just below the removable face plate 20 are a chemical selector valve and an on/off switch 24.
    [036]Chemical Selector Valve 22 allows the user to choose between two or more readily available chemicals. Once the chemical has been selected using the chemical selector valve 22, mode 10 allows the application of the selected chemical mixed with water from the fresh water reservoir 12 through the use of hose 26 and the spray gun applicator 28. Said delivery device consists of a hose 26 and a spray gun 28 extending from the front device of said device 10 between the base 11 and the face plate 20. The spray gun 28 contains two nozzles providing two spray settings allowing the user to select between chemical solution or rinse spray applications.
    [037] When not in use, the hose 26 and spray gun 28 are stored in the hose storage space 30 located at the top of the face plate 20. Located behind and adjacent to the hose storage 30 at the top of the face plate is the removable tool carrier 32. The tool carrier 32 is removable from the base unit and rests on top of the face plate 20. The tool carrier 32 can be used to carry small items such as towels, rags , flannels, small tools, brushes, etc.
    [038] As it is not always feasible or necessary to use the full chemical application capabilities of bucket 10, the present invention provides storage and quick access to handheld cleaning solution spray bottles for small areas of need. Located adjacent to and on either side of the removable tool carrier 32 are two circular storage spaces 34 designed to hold the portable spray bottles.
    [039]Adjacent to both the tool carrier 32 and the storage space 34 are two handle holders 35 one on each side of the face plate designed to hold the tool handles such as mop, brush, broom, etc., while the heads of said tools rest on the base of the figure 10 below the face plate 20.
    [040] Referring now to figure 2, mode 10 is shown in a left rear view. Figure 2 shows a water fill port 50 on the rear side of the base 11 just below the cable 36. The water fill port 50 allows clean water to be poured into the fresh water reservoir 12. Fresh water is poured through. from the water fill port 50 and stored in the fresh water reservoir 12 until it is sprayed as rinse water or combined with chemicals from the chemical storage unit 52 and applied through the hose 26 and the spray gun 28 (Figure 1).
    [041]To increase user efficiency and effectiveness, the present invention allows for the storage and readiness of multiple separate chemical cleaning concentrate materials. Located at the rear of the base 11 just above the water fill port 50 is the chemical storage space 52 containing the chemical concentrate containers 13a,b,c. Chemicals held in chemical storage space 52 remain in their original containers and are connected to mode 10 by removing the shipping cap and seal from each bottle and attaching a chemical feed line to the bottle by screwing the cap on. on the line to the bottle.
    [042] Again referring to Figure 2, it is even more advantageous to increase user efficiency by allowing “one touch” choice between multiple separate cleaning solutions 13a, b, c with the use of a selector switch 22. To this end, embodiment 10 permits placement of multiple chemical concentrate containers 13a, b, c within the chemical storage space 52. Depending on the size of the chemical containers, the space of chemical storage 52 may also allow for the transport of additional chemical containers that are not connected for immediate application use. The multiple active chemical concentrate containers stored in the chemical container space 52 are connected via the chemical supply line and can be selected using a chemical selector valve 22 (Figure 1). Chemicals from chemical storage area 52 are mixed with fresh water from fresh water reservoir 12 and finally distributed through hose 26 and spray gun 28 (Figure 1).
    [043] A primary advantage gained by the present device 10 is the increased mobility and efficiency achieved through the use of a battery 62 (Figure 3) to turn on the pump 60 allowing the user to take advantage of the great advantage achieved when the unit can be operated without relying on or connecting to an external power source.
    [044] The battery 62 is rechargeable via the battery charger 54. In one mode the battery charger 54 is accessed and found on the left side of the base 11 of the unit 10 (Figure 2) in the alternative, the battery charger can be positioned inside the base 11 and outside the external view. By plugging the battery charger 54 into an external power source, the battery shown in Figure 3 can be fully recharged. In the present embodiment 10, the battery charger 54 has two separate rows of lights. The top row indicates battery status. The bottom row of lights indicates charger function. Battery charger 54 is permanently connected to battery 62.
    [045] Referring now to figure 3 a front and right side view of the apparatus 10 is shown with the face plate 20 removed showing only the base 11 of the unit. Removal of face plate 20 allows access to pump 60 and battery 62. Attached to base 11 above fresh water reservoir 12 is pump 60. At the rear of pump 60 is battery 62 which powers the pump.
    [046] Again with reference to Figure 3, pump 60 provides pressure that expels combination of water from freshwater reservoir 12 and chemicals from chemical source containers 52 (Figure 1). The specially calibrated pump provides a low pressure and low volume flow rate and applies the proper amount or dilution of solution while eliminating oversaturation with chemicals and wasted water, chemicals. In a preferred embodiment, the chemical application pressure created by pump 60 and delivered through hose 26 (Figure 1) and spray gun 28 (Figure 1) is about 448.1 to 517.1 kPa ( 65-75 psi), while the pump flow rate is 1/2 gallon per minute. During flush applications the application pressure created by pump 60 is about 689.4-827.3 kPa (100-120 psi). The efficiency advantage provided by the low flow rates is enhanced in the present invention by the high capacity of the freshwater reservoir 12. The low pressure pump 60 and the freshwater reservoir 12 combine to provide up to 28 minutes of run time. without stopping to refuel. Low application and rinse pressure avoids the problems created by higher pressure applicators which, as described above, can force solutions and water into cracks and behind tile work resulting in mold, mildew and the destruction of the connection between the tile work and the floor or wall of the building. As stated above, the low pressure and low volume of a preferred embodiment produces a flow rate of about 1/2 gallon per minute which is about half the volume of prior art devices. And this flow rate is achieved at about 1/3 of the solution applicator pressure against the building surfaces, thus protecting the structure from mold, mildew and damage to the tile. Another benefit is achieved by the low pressure and low volume operation as the same amount of cleaning and the same amount of operator time is involved with the low pressure and low volume device while reducing material waste and the need to clean only half of the applied chemical and/or rinse with water while achieving the same benefit.
    [047] As mentioned above, the present modality operates more silently as it does not include any type of vacuum pickup device like many of the prior art devices. As a result of this change and the use of the low pressure/low volume pump, the present mode operates at just over 65 decibels -- or about the same volume as the sound of a typical conversation -- making the present mode therefore compatible for use in “silence zones” areas such as schools and hospitals.
    [048] In one embodiment the dilution of the chemical concentrate is controlled by the use of specifically sized through tubes or tubes contained within the chemical concentrate bottles. In this way the user is not confronted with the need to calculate dilutions or modify valves or change flow rates to accommodate the different chemicals used with the apparatus 10. Said chemical concentrate bottles having specifically flow-through tubes sized or straws contained within the bottles are known within the art as "Type-F" bottles.
    [049] Referring now to Figure 4, a typical spray gun 28 is depicted which can be used with the invention. Hose inlet 120 attaches to the spray gun in the front cylinder section 122, away from the handle 124 and trigger mechanism 126. The outlet of the spray nozzle receptacle 128 is at the end of the cylinder to which an asper nozzle that is specific for the desired size and flow rate is attached.
    [050] Figure 5 is a typical nozzle attachment including a female body 140, a male body 142, a screen filter 144, a spray tip of desired size and flow rate 146, and a tip retainer 148 that are removable. attached to the outlet of the sprinkling nozzle receptacle.
    [051] The invention is not limited to this specific caddy application system as any pressure spray application system that applies spray less than 517.1 kPa (75 psi) and in accordance with other parameters disclosed in the present invention they are expected to have similar results. Chemical compositions employing proteins
    [052] Proteins such as enzymes form an important part of many cleaning compositions including bathroom sanitizers, floor cleaners and other hard surface cleaners. Any chemical solution that employs protein can be used as long as it is properly diluted in an application/use solution of up to 5 ppm protein can be safely applied in accordance with the invention.
    [053] Enzymes provide desirable activity for protein-based, carbohydrate-based, or triglyceride-based stain removal from substrates; for cleaning, stain removal and sanitization of hard surface cleaners. Enzymes can act by degrading or altering one or more types of dirt residues found on a surface or textile products, thereby removing the dirt or making the dirt more removable by a surfactant or other component of the cleaning composition. Both degradation and alteration of dirt residues can improve detergency by reducing the psychochemical forces that bind the dirt to the surface to be cleaned, ie the dirt becomes more water-soluble. For example, one or more proteases can cleave the complex macromolecular protein structures present in dirt residues into simpler short-chain molecules that are themselves more readily desorbed from surfaces, solubilized or otherwise more easily removed by detersive solutions containing said proteases.
    [054] Compatible enzymes may include a protease, an amylase, a lipase, a gluconase, a cellulase, a peroxidase or a mixture thereof from any compatible origin such as plant, animal, bacterial, fungal or yeast origin. Selections are influenced by factors such as pH activity and/or optimal stability, thermostability and stability for active detergents, builders and the like. In this regard, bacterial or fungal enzymes may be preferred as bacterial amylases and proteases, and fungal cellulases. Preferably, the enzyme can be a Protease, a Lipase, an Amylase or a combination thereof. The enzyme may be present in the applied use solution of up to 5 ppm. In a typical concentrate applied at 2 oz/gal, the concentration could include at least 0.01% by weight to 8% by weight, preferably from about 0.05% by weight to about 5% by weight and, more preferably, from about 0.1% by weight to about 3% by weight.
    [055] Generally chemical cleaning compositions for use in the methods of the invention will be an enzyme stabilizing system. The enzyme stabilizer system can include a boric acid salt such as an alkali metal borate or amine borate (for example an alkanolamine), or an alkali metal borate, borate ester or potassium borate. The enzyme stabilizer system may also include other ingredients to stabilize certain enzymes or to enhance or maintain the effect of the boric acid salt. For example, the cleaning composition for application according to the invention may include a water-soluble source of calcium and/or magnesium ions.
    [056] The enzyme stabilizing components may be present in an amount necessary to stabilize any enzyme present, but will typically be present in an amount from about 0.1% by weight to about 15% by weight, preferably about about 0.5% by weight to about 10% by weight, more preferably from about 1% by weight to about 8% by weight.
    [057] Typical components in said hard surface cleaners include, but are not limited to builders, solvents, surfactants (anionic surfactants, nonionic surfactants, semipolar nonionic surfactants, cationic surfactants, amphoteric surfactants), pH adjusting agents, hydrotopes, defoaming agents, stabilizing agents, chelating/sequestering agents, bleaching agents, anti-redeposition agents, dyes/odorants, divalent ion, polyol, fragrances and/or thickening agents.
    [058] The following is a non-limiting description of examples of components of the invention in addition to the protein that may be present in hard surface cleaning compositions that can be applied in accordance with the. Surfactants
    [059]The sprayable aqueous cleaning composition includes a surfactant. A variety of surfactants can be used including anionic, nonionic, cationic and amphoteric surfactants. Examples of anionic compatible materials are surfactants containing a larger lipophilic moiety and a strong anionic group. Said anionic surfactants typically contain anionic groups selected from the group consisting of sulfonic, sulfuric or phosphoric, phosphonic or carboxylic acid groups which when neutralized will yield a sulfonate, sulfate, phosphonate or carboxylate with a cation in it preferably being selected from the group consisting of of an alkali metal, ammonium, alkanol amine such as sodium, ammonium or triethanolamine. Examples of operative anionic sulfate or sulfonate surfactants include alkylbenzene sulfonates, sodium xylene sulfonates, sodium do-decyl benzene sulfonates, linear sodium tridecylbenzene sulfonates, potassium octyl-decylbenzene sulfonates, sodium lauryl sulfate, palmityl sulfate. sodium, sodium cocoalkyl sulphate, sodium olefin sulphonate.
    [060]Nonionic surfactants do not carry a discrete charge when dissolved in an aqueous media. The hydrophilicity of the non-ionic is provided by the hydrogen bonding with water molecules. Said nonionic surfactants typically comprise molecules containing larger segments of a polyoxyethylene group together with a hydrophobic moiety or a compound comprising a polyoxypropylene and polyoxyethylene segment. Polyoxyethylene surfactants are commonly manufactured through catalyzed ethoxylation based on aliphatic alcohols, alkyl phenols and fatty acids. Polyoxyethylene block copolymers typically comprise molecules having larger segments of ethylene oxide coupled with larger segments of propylene oxide. These nonionic surfactants are well known for use in this area of the art. Additional examples of non-ionic surfactants include alkyl polyglycoside.
    [061] Lipophilic moieties and cationic groups comprising amino or quaternary nitrogen groups can also provide surface-active properties to molecules. As the name implies for cationic surfactants, the hydrophilic half of nitrogen carries a positive charge when dissolved in aqueous media. The soluble surfactant molecule can have its solubility or other surfactant properties increased by using alkyl groups or low molecular weight alkyl hydroxyl groups.
    [062]The cleaning composition may contain a cationic surfactant component which includes a detersive amount of cationic surfactant or a mixture of cationic surfactants. Cationic surfactant can be used to provide sanitizing properties. In one example, cationic surfactants can be used in basic compositions.
    [063] Cationic surfactants that can be used in the cleaning composition include, but are not limited to: amines such as primary, secondary and tertiary monoamines with alkyl or alkenyl chains, ethoxylated alkylamines, ethylenediamine alkoxylates, imidazoles such as 1-( 2-hydroxyethyl)-2-imidazoline, 2-alkyl-1-(2-hydroxyethyl)-2-imidazoline, and the like; and quaternary ammonium compounds and salts, such as alkylquaternary ammonium chloride surfactants such as n-(C12-C18)alkyl dimethylbenzyl ammonium chloride, n-tetradecyldimethylbenzylammonium chloride monohydrate, a naphthylene substituted quaternary ammonium chloride as dimethyl chloride -1-naphthylmethylammonium.
    [064]Amphoteric surfactants can also be used. Amphoteric surfactants contain both acidic and basic hydrophilic halves in the structure. These ionic functions can be any of the anionic or cationic groups that have been previously described in the sections related to anionic and cationic surfactants. Briefly, anionic groups include carboxylates, sulfates, sulfonates, phosphonates, etc. while cationic groups typically comprise compounds having amine nitrogen. Many amphoteric surfactants also contain ether oxides or hydroxyl groups which enhance their hydrophilic tendency. Preferred amphoteric surfactants of this invention comprise surfactants having a cationic amino group combined with an anionic carboxylate or sulfonate group. Examples of useful amphoteric surfactants include sulfobetaines, N-coco-3,3-aminopropionic acid and its sodium salts, n-tallow-3-amino-dipropionate, disodium salts, 1,1-bis(carboxymethyl)-2-undecyl -2-imidazolinium hydroxide disodium salts, cocoaminobutyric acid, cocoaminopropionic acid, cocoamidocarboxy glycinate, cocobetaine. Compatible amphoteric surfactants include cocoamidopropylbetaine, polyether siloxane, and cocoaminoethylbetaine.
    [065]Amine oxides, such as tertiary amine oxides, can also be used as surfactants. Tertiary amine oxide surfactants typically comprise three alkyl groups attached to an amine oxide (N^O). Commonly the alkyl groups comprise two lower (C1-4) alkyl groups combined with a C6 -24 higher alkyl group, or it may comprise two higher alkyl groups combined with a lower alkyl group. In addition, lower alkyl groups may comprise alkyl groups substituted with hydrophilic moiety such as amine hydroxyl groups, carboxylic groups, etc. compatible amine oxide materials include dimethylcetylamine oxide, dimethyllaurylamine oxide, dimethylmyristylamine oxide, dimethylstearylamine oxide, dimethylcocoamine oxide, dimethyldecylamine oxide, and mixtures thereof. The classification of amine oxide materials may depend on the pH of the solution. On the acid side, amine oxide materials protonate and can simulate characteristics of cationic surfactants. At neutral pH, amine oxide materials are non-ionic surfactants and on the alkaline side they exhibit anionic characteristics.
    [066] Another important class of surfactants includes functionalized alkyl polyglycosides which may fall into the class of surfactants depending on functional groups (nonionic, anionic, amphoteric etc.). An example includes the “green” series of surfactants based on the renewable resources of alkyl polyglycosides available from Colonial Chemical. These include alkyl polyglycoside derivatives with various functional groups such as sulfonated and polysulfonated alkyl polyglycoside derivatives, alkyl phosphate or polyphosphate polyglycoside derivatives, quaternary alkyl functionalized polyglycoside derivatives, polyquaternary alkyl functionalized polyglycoside derivatives, thaina alkyl functionalized polyglycoside derivatives , alkyl-functionalized polyglycoside sulfosuccinate derivatives, and the like.
    [067] The surfactant is present in the composition in an amount of from about 1% by weight to about 60% by weight, from about 5% by weight to about 55% by weight and from about 10% by weight to about 50% by weight. builders
    [068] Detergency builders useful in liquid compositions include alkali metal silicates, alkali metal carbonates, polyphosphonic acids, C10-C18 alkyl monocarboxylic acids, polycarboxylic acids, alkali metal, ammonium or substituted ammonium salts thereof, and mixtures of the same.
    [069] The builder is preferably present in the composition in an amount of from about 0 to about 8% by weight, from about 0.01 to about 5% by weight and from about 0.05 to about 2% by weight. pH adjustment compound
    [070] The compositions of the present invention have a pH of from about 4.0 to about 8. Within that pH range, the present compositions effectively reduce microbial populations and are acceptable to the consumer, i.e., they are mild for the skin, they are phase stable, and generate abundant stable foam. In some cases a pH adjusting compound may be needed in an amount sufficient to provide a desired composition pH. To achieve the full advantage of the present invention, the pH adjusting compound is present in an amount of from about 0.05% to about 3.5% by weight.
    [071]Examples of basic pH adjusting compounds include, but are not limited to ammonia; mono-, di- and trialkylamines; mono-, di-, and trialkanolamines; alkali metal and alkaline earth metal hydroxides; alkali metal phosphates; alkaline sulfates; alkali metal carbonates and mixtures thereof. However, the identity of the basic pH adjuster is not limited and any basic pH adjuster compound known in the art can be used. Specific non-limiting examples of pH adjusting compounds are ammonia, sodium, potassium and lithium hydroxide; sodium and potassium phosphates, including hydrogen and dihydrogen phosphates; sodium and potassium carbonate and bicarbonate; sodium and potassium sulfate and bisulfate; monoethanol amine; trimethylamine; isopropanolamine; diethanol amine; and triethanol amine.
    [072] The identity of a pH adjusting compound is not limited to any acidic pH adjusting compound known in the art, alone or in combination, can be used. Examples of specific acidic pH adjusting compounds are mineral acids and polycarboxylic acids. Non-limiting examples of mineral acids are hydrochloric acid, nitric acid, phosphoric acid and sulfuric acid. Non-limiting examples of polycarboxylic acids are citric acid, glycolic acid and lactic acid. The pH adjusting agent is present if necessary, but is generally present in the composition in an amount of from about 0 to about 5% by weight, from about 0.01 to about 3% by weight, and from about 0.01 to about 3% by weight. about 0.05 to about 2% by weight. Solvent
    [073]A solvent is often used in cleaning compositions to improve their dirt removal properties. The cleaning compositions of the invention can include a solvent to adjust the viscosity of the final composition. The intended end use of the composition can determine whether or not the solvent is included in the cleaning composition. If a solvent is included in the cleaning composition, it is usually a low-cost solvent such as isopropyl alcohol. A solvent may or may not be included to improve dirt removal, hood capability or ease of use of the compositions of the invention. Compatible solvents useful in removing hydrophobic soils include, but are not limited to: oxygenated solvents such as lower alkanols, lower alkyl ethers, glycols, aryl glycol ethers and lower alkyl glycol ethers. Examples of other solvents include, but are not limited to: methanol, ethanol, propanol, isopropanol and butanol, isobutanol, ethylene glycol, diethylene glycol, triethylene glycol, propylene glycol, dipropylene glycol, mixed ethylene-propylene glycol ethers, ethylene ether phenyl glycol, and propylene glycol phenyl ether. Substantially water soluble glycol ether solvents include, but are not limited to: propylene glycol methyl ether, propylene glycol propyl ether, dipropylene glycol methyl ether, tripropylene glycol methyl ether, ethylene glycol butyl ether, diethylene glycol methyl ether, diethylene glycol butyl ether, ethylene glycol dimethyl ether, ethylene glycol propyl ether, diethylene glycol ethyl ether, triethylene glycol methyl ether, triethylene glycol ethyl ether, triethylene glycol butyl ether and the like.
    [074] The solvent is preferably present in the composition in an amount of from about 0.1 to about 18% by weight, from about 0.5 to about 10% by weight and from about 1 to about 8% by weight. Defoamer
    [075] A smaller but effective amount of a defoaming agent to reduce foam stability may also be included in the compositions. The cleaning composition may include 0.01-5 % by weight of a defoamer or 0.01-3 % by weight.
    [076]Examples of defoaming agents include silicone compounds such as silica dispersed in polydimethylsiloxane, fatty amides, hydrocarbon waxes, fatty acids, fatty esters, fatty alcohols, fatty acid soaps, ethoxylates, mineral oils, polyethylene glycol esters, alkyl phosphate such as monostearyl phosphate and the like. A discussion of defoaming agents can be found, for example, in US Patent No. 3,048,548 to Martin et al., US Patent No. 3,334,147 to Brunelle et al., and US Patent No. 3,442,242 to Rue et al. Al., the disclosures being incorporated herein by reference. The defoaming agent is preferably present in the composition in an amount of from about 0 to about 5% by weight, from about 0.01 to about 3% by weight, and from about 0.05 to about 2% by weight. . water conditioning agent
    [077]Water conditioning agents help to remove metal compounds and reduce harmful effects of hard components in service water. Exemplary water conditioning agents include chelating agents, sequestering agents, and inhibitors. The cations of polyvalent metals or compounds such as calcium, magnesium, iron, manganese, molybdenum, etc. cation or compounds, or mixtures thereof, may be present in service water and complex dirt. Said compounds or cations can interfere with the effectiveness of a washing or rinsing composition during cleaning application. A water conditioning agent can effectively be complex and remove said compounds or cations from soiled surfaces and can reduce or eliminate inappropriate interaction with active ingredients including the nonionic surfactants and anionic surfactants of the invention. Both organic and inorganic water conditioning agents are common and can be used. Inorganic water conditioning agents include compounds such as sodium tripolyphosphate and other species of larger linear and cyclic polyphosphates. Organic water conditioning agents include both small molecule and polymeric water conditioning agents. Small molecule organic water conditioning agents are typically organocarboxylate compounds or organophosphate water conditioning agents. Polymeric inhibitors commonly comprise polyanionic compositions such as polyacrylic acid compounds. Small molecule organic water conditioning agents include, but are not limited to: sodium gluconate, sodium glucoheptonate, N-hydroxyethylenediaminetriacetic acid (HEDTA), ethylenediaminetetraacetic acid (EDTA), nitrilotriacetic acid (NTA), acid diethylenetriaminepentacetic acid (DTPA), ethylenediaminetetrapropionic acid, triethylenetetraminehexaacetic acid (TTHA), and its alkali metal, ammonium or substituted ammonium salts thereof, ethylenediaminetetraacetic acid tetrasodium salts (EDTA), nitrilotriacetic acid trisodium salts (NTA) , ethanoldiglycine disodium salts (EDG), diethanolglycine sodium salt (DEG), and 1,3-propylenediaminetetraacetic acid (PDTA), dicarboxymethyl glutamic acid tetrasodium salts (GLDA), methylglycine-NN-diacetic acid trisodium salts (MGDA), and sodium salt iminodisuccinate (IDS). All of these are known and commercially available. The defoaming agent is preferably present in the composition in an amount of from about 0 to about 15% by weight, from about 0.01 to about 10% by weight and from about 0.05 to about 5% by weight. hydrotopes
    [078] Compositions of the invention may optionally include a hydrotope that aids in compositional stability and aqueous formulation. Speaking of functionality, the compatible hydrotope couplers that can be employed are non-toxic and retain the active ingredients in the aqueous solution over the entire temperature and concentration range to which a concentration or any use solution is exposed.
    [079]A hydrotope coupler can be used as long as it does not react with the other components of the composition or negatively affect the performance properties of the composition. Representative classes of hydrotopic coupling agents or solubilizers that may be employed include anionic surfactants such as alkyl sulfates and alkane sulfonates, linear alkyl naphthalene or benzene sulfonates, secondary alkane sulfonates, alkyl ether sulfonates or sulfates , alkyl phosphonates or phosphates, di-alkyl sulphosuccinic acid esters, sugar esters (eg sorbitan esters), amine oxides (mono-, di-, or tri-alkyl) and C8-C10 alkyl glucosides . Preferred coupling agents for use in the present invention include n-octane sulfonate, available as NAS 8D from Ecolab Inc., n-octyl dimethylamine oxide, and commonly available aromatic sulfonates such as alkyl benzene sulfonates (e.g. xylene) or naphthalene sulfonates, aryl or alkaryl phosphate esters or their alkoxylated analogues having 1 to about 40 ethylene, propylene or butylene oxide units or mixtures thereof. Other preferred hydrotopes include C6-C24 alcohol alkoxylate nonionic surfactants (alkoxylate means ethoxylates, propoxylate, butoxylate and mixtures of co- or terpolymers thereof) (preferably C6-C14 alcohol alkoxylates) having 1 to about 15 groups of alkylene oxide (preferably about 4 to about 10 alkylene oxide groups); C6-C24 alkylphenol alkoxylates (preferably C8-C10 alkylphenol alkoxylates) having 1 to about 15 alkylene oxide groups (preferably about 4 to about 10 alkylene oxide groups); C6-C24 alkylpolyglycosides (preferably C6-C20 alkylpolyglycosides) having 1 to about 15 glycoside groups (preferably about 4 to about 10 glycoside groups); C6-C24 fatty acid ester ethoxylates, propoxylates or glycerides; and C4-C12 mono or dialkanolamides. A preferred hydrotope is sodium xylene sulfonate (SXS). The composition of an optional hydrotope can be present in the range of from about 0 to about 25 percent by weight. Vehicles
    [080]The cleaning composition also includes water as a vehicle. It should be appreciated that water can be supplied as deionized water or as softened water. Water supplied as part of the concentrate may be relatively free of hardness. It is expected that the water can be deionized to remove a portion of the dissolved solids. That is, the concentrate can be formulated with water that includes dissolved solids and can be formulated with water that can be characterized as hard water. The compositions may include in a concentrate from about 40% by weight to about 90% by weight of water, from about 45% by weight to about 85% by weight and from about 50% by weight to about 80% % by weight.
    Compositions comprising protein are typically hard surface disinfecting or cleaning compositions and are designed for a spray and let or spray and dry mode of application.
    [082] In said applications, the user generally applies an effective amount of the composition using the pump and within a few moments thereafter, dries the treated area with a cloth, towel or sponge usually a disposable sponge or paper towel. In certain applications, however, especially where unwanted stain deposits are heavy, such as grease stains, the cleaning composition according to the invention may be left on the stained area until it has effectively dislodged the stain deposits after which time this can then be dried, rinsed, or otherwise removed. For particularly heavy deposits of said unwanted stains, various applications can also be used. Optionally, after the composition has remained on the surface for a period of time, it can be rinsed or dried from the surface. Due to the viscoelasticity of the compositions, cleaning compositions have improved adhesion and remain for long periods of time even on vertical surfaces.
    [083] While compositions for use of the methods of the invention are generally discussed and exemplified in concentrated types of liquid forms described, nothing in this specification should be construed as limiting the use of the composition according to the invention with another amount of water to form a cleaning use solution from there. In said proposed diluted cleaning solution, the greater the proportion of water added to form said cleaning dilution, the greater can be the reduction in rate and/or effectiveness of the so formed cleaning solution. Consequently, longer residence times after the stain affects its detachment and/or the use of larger amounts may be necessary. Preferred dilution ratios of the concentrated hard surface cleaning composition:water of 1:1-200, preferably 1:2-100, more preferably 1:3-100, even more preferably 1:10-100, and most preferably 1:16-85, in two weight/weight (“w/w”) ratios or alternatively in a volume/volume (“v/v”) ratio.
    [084] On the other hand, nothing in the specification should be construed as limiting the formation of a “superconcentrated” cleaning composition based on the composition described above. Said superconcentrate ingredient composition is essentially the same as the cleaning compositions described above except that it includes a smaller amount of water. Typical no-rinse floor cleaning composition
    [085] By way of example, a typical protein containing no-rinse floor cleaner composition to be used in the method of the invention is below: Typical no-rinse floor cleaner 2nd variety 3rd variety % by weight % by weight % by weight
    Typical sanitizing rinse-off floor cleaning composition 1st variety 2nd variety 3rd variety % by weight % by weight % by weight
    Methods Employing Compositions
    [086] Again with reference to Figure 3, pump 60 provides pressure that expels combination of water from freshwater reservoir 12 and chemicals from chemical source containers 52 (Figure 1). The specially calibrated pump provides a low pressure and low volume flow rate and applies the proper amount or dilution of solution while eliminating oversaturation with chemicals and wasted water, chemicals. In a preferred embodiment, the chemical application pressure created by pump 60 and distributed through hose 26 (Figure 1) and spray gun 28 (Figure 1) is about 448.1 to 517.1 kPa (65-75 psi), preferably at 517.1 kPa (75 psi) and not greater when pump flow rates are 1/2 gallon per minute. During rinse applications the application pressure created by pump 60 is about 689.4-827.3 kPa (100-120 psi). The efficiency advantage provided by the low flow rates is enhanced in the present invention by the high capacity of the freshwater reservoir 12. The low pressure pump 60 and the freshwater reservoir 12 combine to provide up to 28 minutes of time. running without stopping to refuel. Any means can be used to apply the compositions provided the critical dilution, pressure ratio and particle size are achieved. This could include a sprinkler at the end of the garden hose, for example.
    [087]Low application pressure avoids the problems created by higher application pressure which, as described above, is one of the factors that prevent proteins from becoming aerosolized and thus improve safety. Higher pressure can also cause additional problems as it can force the solutions and water into the cracks and behind the tiles and result in mold, mildew and the destruction of the connection between the tile and the floor or wall of the building. As stated above, the low pressure and low volume of a preferred embodiment produces a flow rate of about 1/2 gallon per minute which is about half the flow rate of prior art devices. This flow rate is achieved at about 1/3 of the application pressure of the solution against the building surfaces, thus protecting the user from protein aerosolization. Examples
    [088] The present invention is more particularly described in the following examples which are intended for illustration only, since various modifications and variations within the scope of the present invention will be apparent to those skilled in the art. Except where otherwise specified, all parts, percentages and ratios given in the following examples are on a weight basis, and all reagents used in the examples were obtained, or are available from, the chemical suppliers described below or can be synthesized by conventional techniques.
    [089] The formulations were prepared according to the tables below: Standard No Rinse Floor Cleaner 1st Variety 2nd Variety 3rd Variety % by weight % by weight % by weight
    Sanitizing floor cleaner 1st variety 2nd variety 3rd variety % by weight % by weight % by weight
    Anti-fog floor cleaner 1st assortment 2nd assortment 3rd assortment % by weight % by weight % by weight


    [090]The anti-fog agent is Dow chemical's Poliox WSR-301 (high molecular weight poly(ethylene oxide) polymer).
    [091]The amount of solvent was used twice in the Anti-Mist Floor Cleaner Formula in order to keep the polyox stable and in solution. Different measuring tips were evaluated to achieve the desired dilution due to the anti-fog formula being thicker and more difficult to dispense. Example 1
    [092]Determination of Anti-Mist Floor Cleaner Measuring Tip for Caddy Test Purpose
    [093]The assigned values assigned to the measuring tips are only guaranteed with fine water products. Sanitizers and standard no-rinse floor cleaners were based on the measuring tip chart as they were fine water. This test was done to determine which measuring tip is suitable for dispensing 2 oz/gal of anti-fog enhanced cleaning solutions. measuring tips
    [094]The following chart is to be used as a guide. The list shows holes in ascending order from smallest (Brown) to largest (Black).

    Procedure 1) Samples prepared one day before testing to ensure fresh polyox. 2) RMs added with the mixture so that they appear in the above formulas, except for the improved polyox solution. Poliox was pre-mixed with propylene glycol and added at the end. 3) No enzyme was included in the test 4) After the polyox was added, the solution was set up on a stir plate and mixed for ~ 1 h at 200 rpm until the polyox had completely entered the solution. 5) Day of polyox test to be added to caddy-specific pouches. 6) Solution bag placed in caddy and injected through sprinkler so that solution passes through all tubes. 7) Solution pouch removed from caddy, weighed and placed back in caddy. The solution was sprayed for 1:30 in a collection tube. 8) The bag of solution removed and re-weighed to calculate the amount of solution used. The tube is weighed to calculate the amount of solution dispensed. 9) A percent concentrate to RTU dispensed is calculated to give a percent concentration and compared to 2 oz/gal (1.56%). 10) The measuring tips are changed multiple times to determine which will give us the desired concentration of 1.56% improved polyox solution dispensed. Data
    [095]The goal of the test is to find a measuring tip that is capable of dispensing 1.56% Poliox concentrate (2 oz/gal). The measuring tip for the anti-mist formula was determined using the standard spray nozzle. The data below is from the concentrated Polyox test only. Internal Transport

    [096]The proper tip according to the results found in our four tests for the concentrated polyox will be a brown measuring tip using standard sprinklers. Example 2
    [097]The experiments were carried out in an attempt to reduce the aerosolization of proteins from the solution applied in commercial caddy cleaning systems. The cleaning caddy has a spray device that is used to apply various non-enzymatic cleaners to hard surfaces at an average pressure of 482.6 kPa (70 psi). In this evaluation the enzymatic cleaner is mixed with the water at a ratio of 2 oz/gal (15.6 ml/l) before being sprayed onto the tile at a flow rate of 1/2 gallon/min (1.9 l/min). The undiluted product contains 1% Lipex 100L (Novozymes)
    [098] An experiment was carried out to assess the amounts of aerosolized enzymes that the person operating the cleaning caddy will be exposed to.
    [099] The experiment was carried out while using a commercial caddy system as described herein, product and three formulations, a standard no-rinse formulation, a sanitizing cleaning composition and an anti-fog formulation. These formulations have been applied using the existing spray device. All product formulations are liquid and contain Lipez 100 to 1% (v/v). The cleaning caddy has a wet vacuum machine creation. Exposure has been assessed during product removal using this wet vacuum machine as well as using a squeegee. The evaluation is focused on determining the peak exposure generated by each application, but also on a monitoring average in relation to the cleaning cycle as a whole has been determined. Final total results
    [0100]The results are summarized in Table 1 Table 1 Exposure to Lipex during all relevant cleaning handling processes for the three different Caddy formulations. All exposure data were given as: ng active enzyme protein/m3 air.
    Enzyme Exposure Sampling
    [0101] Enzyme exposure assessment was performed on these different combinations: 1. The commercial spray caddy cleaning formulation being sprayed, followed by scrubbing with a stiff bristle brush and removed by a wet vacuum. 2. Commercial spray caddy sanitizing formulation being sprayed, followed by scrubbing with a stiff bristle brush and squeegeeing. 3.Commercial anti-mist spray caddy formulation being sprayed, followed by scrubbing with a stiff bristle brush and removed by a wet vacuum.
    [0102]To determine if there is any exposure of the machine to vacuum, additional air samples were taken near the exhaust pipe.
    [0103]During the evaluation two Gillian Aircon pumps were used to determine the exposure of the cleaning cycle as a whole and two were used to evaluate each individual application ie spray, scrub, squeegee drying or wet vacuum. To keep the filters within one meter of the operator's breathing zone throughout the full monitoring time they were mounted on two carts which were kept on either side of the operator. Filters were positioned 150 cm above the floor. To avoid biased results each caddie has a full cycle sampling pump and an individual process sampling pump, in the caddies the left pump was sampled throughout the cycle and the right one was sampled during the single application.
    [0104] Each enzyme exposure sampling was performed according to the procedure set out below:
    Materials and Methods Air Sampling Four Gillian AirCon pumps were used.
    [0105]All samples were taken with an air flow of 25 liters per minute within one meter of the operators breathing zone. Sampling time was recorded and filters stored at -20°C until analysis. Samples
    [0106]38 air filters were collected, stored and frozen until analyzed. Filter samples
    [0107] Filters were eluted during shaking in 5 mL PBS/BSA/Brij (0.01 M Phosphate / 0.5% BSA / 0.023% Brij (active surface ingredient) buffer pH 7.4 for 30 min. Essay
    [0108]The enzyme-specific protein analyzes were performed by ELISA. All samples were analyzed for Lipex. An enzyme protein standard curve was analyzed on each microtiter plate. Samples were analyzed in a 2-fold dilution series in duplicate, samples that gave unreliable results were analyzed again the following day. Enzyme exposure was calculated for each filter. Results
    [0109]Adsorbed enzyme was eluted on filters used during the enzyme exposure assessment. This was subsequently analyzed using ELISA technology. Detailed exposure data are found in Table 2. Discussion sprinkling
    [0110]Enzyme exposure data show that spraying with the standard spray nozzle results in exposures between 24 and 31 ng/m3. Brushing
    [0111]The enzyme exposure during brushing was determined four times and showed exposure below the detection limit in all these measurements. Removal of wet vacuum from the product
    [0112]In two cleaning cycles the product was removed from the floor using the wet vacuum system that is installed in the caddy. For the two products (standard cleaning composition and anti-mist formulation) that were applied using the normal spray nozzle the exposure was below the detection limit, 1.42 ng active enzyme protein/m3.
    [0113] This evaluation was made using the Formulations described above with the product being applied to the floor. In order to make this assessment a set of filters was mounted close to the exhaust pipe, pumps were started and the product was removed according to the same procedure as before. Enzyme exposure was below detection limit. Product removal with a squeegee
    [0114]Product was also removed using a squeegee to determine exposure when cleaning solution is removed through floor drain. The exposure of this application was determined to be 1.42 ng active enzyme protein/m3. Average exposure over the full cycle
    [0115]The exposure measurements taken in relation to the entire cleaning cycle are consistent with the exposure of the individual measurements. All three formulas have an individual process that generates significantly higher exposure than other individual processes and thus this is the biggest contributor to the average exposure. In this exposure assessment we are focusing on the exposure peaks that are generated during each specific cleaning process.
    [0116] Enzyme allergies can develop when humans are exposed to active enzyme protein through inhalation. Routes of exposure are via aerosolized enzyme proteins or enzyme dusts. Due to REACH legislation in the EU, a Derived Minimum Effect Content (DMEL) for enzymes has been adopted throughout the enzyme industry and the detergent industry as a guide. The DMEL describes threshold values for enzyme exposure and when exposure is kept below this level, the risk of developing allergy is very low. The corresponding DMEL for occupational exposure is set at 60 ng/m3 as peak exposure.
    [0117] Outside the EU the ACGIH Limit Value of 60 ng/m3 for peak occupational exposure is applied in most countries. However, UK authorities have installed an additional Limit Value of 40 ng/m3 for the average 8 hour occupational exposure. Conclusion
    [0118]The proper measuring tips have been determined for the standard sprinkler on the caddy that dispenses the correct amount of 1.56% polyox solution (2 oz/gal). When comparing polyox and non-polyox solutions through each sprinkler, no significant difference was seen in the sprinkler or anti-fog pattern. Polyox is added to solutions to increase particle size and is a traditional mechanism in an attempt to reduce protein aerosolization. Quite surprisingly, applicants have found that aerosolization can be better controlled without any additives and via the spray parameters discussed here. The addition of Poliox does not result in any significant difference in aerosolization. Table 2

    Table 2 (cont.)

    权利要求:
    Claims (21)
    [0001]
    1. Method for applying protein wear solutions to prevent their aerosolization, CHARACTERIZED by the fact that it comprises: placing a hard surface to be cleaned in contact with a wear solution comprising up to 5 ppm protein and 0.1% by weight to 25% by weight surfactant, wherein the composition is free of anti-mist components and wherein contact occurs under pressure conditions of not more than 689.4 kPa (100 psi); and allowing the wear solution to dry, or removing the wear solution from the surface.
    [0002]
    2. Method according to claim 1, CHARACTERIZED in that the removal of said wearing solution from said hard surface is by wiping said hard surface so that dirt and debris on said hard surface are removed with the use solution.
    [0003]
    3. Method according to claim 1, CHARACTERIZED by the fact that said protein is lipase.
    [0004]
    4. Method according to claim 1, CHARACTERIZED by the fact that said step of putting in contact is through a pressurized spray system.
    [0005]
    5. Method according to claim 4, CHARACTERIZED by the fact that said pressurized spray system includes a spray trigger nozzle.
    [0006]
    6. Method according to claim 5, CHARACTERIZED by the fact that said spray trigger nozzle has a pressure of not more than 689.4 kPa (100 psi) at a flow rate of 3.79 liters per minute (1 gallon per minute).
    [0007]
    7. The method of claim 5, CHARACTERIZED by the fact that said spray trigger nozzle has a pressure of no more than 517.1 kPa (75 psi) at a flow rate of 2.84 liters per minute (0.75 gallons per minute).
    [0008]
    8. The method of claim 5, CHARACTERIZED by the fact that said spray trigger nozzle has a pressure of no more than 517.1 kPa (75 psi) at a flow rate of 1.89 liters per minute (0.5 gallons per minute).
    [0009]
    9. Method according to claim 6, CHARACTERIZED by the fact that said flow rate is up to 850.5 grams (30 ounces) of the use solution per minute.
    [0010]
    10. Method according to claim 4, CHARACTERIZED by the fact that said pressurized spray system generates a particle size of the use solution from 675 to 1500 microns.
    [0011]
    11. The method of claim 1, CHARACTERIZED in that it further comprises: adding a chemical concentrate to a pressurized spray application system comprising: (a) a reservoir adapted to store fresh water; (b) a spray tool fluidly communicating with said reservoir; and (c) a spray pump fluidly communicating with said reservoir, said chemical concentrate container, and said spray tool for mixing the chemical concentrate and fresh water to form the use solution and boosting the said use solution outside the perfusion tool by activating said pump; applying said wearing solution through the spray tool at a pressure of not more than 689.4 kPa (100 psi), the wearing solution having a particle size of at least 750 microns; and removing said wearing solution from said surface allowing the wearing solution to evaporate or drying said wearing solution from said surface.
    [0012]
    12. Method according to claim 11, CHARACTERIZED by the fact that said proteins are present in the use solution at a concentration of 60 ng/m3 or less.
    [0013]
    13. Method according to claim 11, CHARACTERIZED by the fact that said chemical concentrate includes up to 3% by weight of protein.
    [0014]
    14. Method according to claim 11, CHARACTERIZED by the fact that said protein is lipase.
    [0015]
    15. The method of claim 11, CHARACTERIZED by the fact that the chemical concentrate is diluted to a dilution of 56.7 g (2 ounces) of chemical concentrate per 3.69 liters (gallon) of solution of use.
    [0016]
    16. Method according to claim 11, CHARACTERIZED by the fact that said chemical concentrate includes up to 5% by weight of protein.
    [0017]
    17. Method according to claim 1, CHARACTERIZED by the fact that the anti-mist components are selected from the group of polyethylene oxide, polyacrylamide, polyacrylate, and combinations thereof.
    [0018]
    18. Method according to claim 1, CHARACTERIZED by the fact that the surfactant is selected from the group of anionic surfactants, non-anionic surfactants, cationic surfactants, amphoteric surfactants, and mixtures of these.
    [0019]
    19. Method according to claim 1, CHARACTERIZED by the fact that the surfactant is selected from the group of: alkylbenzene sulfonates, sodium xylene sulfonates, sodium dodecyl benzene sulfonates, linear sodium tridecyl-benzene sulfonates, octyldecylbenzene sulfonates of potassium, sodium lauryl sulphate, sodium palmityl sulphate, sodium cocoalkyl sulphate, sodium olefin sulphonate, C6-24 alcohol ethoxylates, alkylpolyglycosides, ethoxylated alkylamines, ethylenediamine alkoxylates, 1-(2-hydroxyethyl)- 2-imidazoline, a 2-alkyl-1-(2-hydroxyethyl)-2-imidazoline, n-alkyl(C12-C18)dimethylbenzyl ammonium chloride, n-tetradecyldimethylbenzylammonium chloride monohydrate, dimethyl-1-naphthylmethylammonium chloride, sulfobetaines, N-coco-3,3-aminopropionic acid, n-tallow-3-amino-dipropionate disodium salt, 1,1-bis(carboxymethyl)-2-undecyl-2-imidazolinium hydroxide disodium salt, cocoaminobutyric acid, cocoaminopropionic acid, cocoamidocar glycinate boxi, cocobetaine, cocoamidopropylbetaine, polyether siloxane, cocoaminoethylbetaine, dimethylcetylamine oxide, dimethyllaurylamine oxide, dimethylmyristylamine oxide, dimethylstearylamine oxide, dimethylcocoamine oxide, dimethyldecylamine oxide, and mixtures thereof.
    [0020]
    20. Method according to claim 1, CHARACTERIZED by the fact that the use solution further comprises an additional ingredient selected from the group of a pH modifier, an enzyme stabilizer, a water conditioning agent, a hydrotope, and mixtures thereof.
    [0021]
    21. Method according to claim 20, CHARACTERIZED by the fact that the pH modifier is selected from the group of hydrochloric acid, nitric acid, phosphoric acid, sulfuric acid, citric acid, glycolic acid, lactic acid, and mixtures of the same.
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    法律状态:
    2020-01-14| B06U| Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]|
    2021-01-26| B06A| Patent application procedure suspended [chapter 6.1 patent gazette]|
    2021-06-29| B09A| Decision: intention to grant [chapter 9.1 patent gazette]|
    2021-08-03| B350| Update of information on the portal [chapter 15.35 patent gazette]|
    2021-08-31| B16A| Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]|Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 27/04/2015, OBSERVADAS AS CONDICOES LEGAIS. |
    优先权:
    申请号 | 申请日 | 专利标题
    US14/263,003|US10119101B2|2014-04-28|2014-04-28|Method of minimizing enzyme based aerosol mist using a pressure spray system|
    US14/263,003|2014-04-28|
    PCT/US2015/027853|WO2015168040A1|2014-04-28|2015-04-27|Method of minimizing enzyme based aerosol mist using a pressure spray system|
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